sabato 16 agosto 2014

RUO real-time PCR protocols without nucleic acid purification

for DNA viruses : AmpDirect (Shimadzu/Nacalai); HSV (ref), HBV (ref)

for RNA viruses : Cells-to-CT(TM) 1-Step Power SYBR(R) Green Kit (Life Technologies)

Since haemolysed, lipaemic and icteric serum samples from patients are inevitable in clinical practice, investigators should evaluate the interference of these endogenous substances (also known as the serum index), including conjugated bilirubin, haemoglobin and triglycerides, in real-time PCR.
Avoiding DNA extraction reduces risk of cross contamination, carryover and/or procedural error.

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